enhancement of plant metabolite fingerprinting by machine
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Enhancement of plant metabolite fingerprinting by machine ...

Metabolite fingerprinting of Arabidopsis (Arabidopsis thaliana) mutants with known or predicted metabolic lesions was performed by (1)H-nuclear magnetic resonance, Fourier transform infrared, and flow injection electrospray-mass spectrometry. Fingerprinting enabled processing of five times more plan

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Enhancement of Plant Metabolite Fingerprinting by Machine ...

2010-6-21  Metabolite fingerprinting of Arabidopsis (Arabidopsis thaliana) mutants with known or predicted metabolic lesions was performed by 1 H-nuclear magnetic resonance, Fourier transform infrared, and flow injection electrospray-mass spectrometry. Fingerprinting enabled processing of five times more plants than conventional chromatographic profiling ...

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Enhancement of Plant Metabolite Fingerprinting by

Bioinformatics Enhancement of Plant Metabolite Fingerprinting by Machine Learning1[wl Ian M. Scott*, Cornelia P. Vermeer, Maria Liakata, Delia I. Corol, Jane L. Ward, Wanchang Lin,

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Enhancement of Plant Metabolite Fingerprinting by Machine ...

2010-7-31  Fingerprinting enabled processing of five times more plants than conventional chromatographic profiling and was competitive for discriminating mutants,

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Enhancement of plant metabolite fingerprinting by machine ...

Metabolite fingerprinting of Arabidopsis (Arabidopsis thaliana) mutants with known or predicted metabolic lesions was performed by 1H-nuclear magnetic resonance, Fourier transform infrared, and flow injection electrospray-mass spectrometry. Fingerprinting enabled processing of five times more plants than conventional chromatographic profiling and was competitive for discriminating mutants ...

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Enhancement of Plant Metabolite Fingerprinting by Machine ...

An alternative that prioritizes speed over metabolite identification is metabolite (or metabolic) "fingerprinting," whose minimalist versions have little sample preparation and no chromatography (Hall, 2006). We compared three primary spectroscopies in analyses of unfractionated plant extracts.

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Enhancement of Plant Metabolite Fingerprinting by Machine ...

Enhancement of Plant Metabolite Fingerprinting by Machine Learning1[W] By Ian M ... and flow injection electrospray-mass spectrometry. Fingerprinting enabled processing of five times more plants than conventional chromatographic profiling and was competitive for discriminating mutants, other than those affected in only low-abundance metabolites ...

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Open Research: Enhancement of Plant Metabolite ...

2017-4-6  Metabolite fingerprinting of Arabidopsis (Arabidopsis thaliana) mutants with known or predicted metabolic lesions was performed by 1H-nuclear magnetic resonance, Fourier transform infrared, and flow injection electrospray-mass spectrometry.

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Metabolite fingerprinting: detecting biological features ...

Motivation: Metabolite fingerprinting is a technology for providing information from spectra of total compositions of metabolites. Here, spectra acquisitions by microchip-based nanoflow-direct-infusion QTOF mass spectrometry, a simple and high throughput technique, is tested for its informative power.

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The enhancement of plant secondary metabolites content in ...

2020-2-28  Plant secondary metabolites (PSM) are natural sources of biologically active compounds used for a healthy diet, in traditional medicine and in a wide range of industrial applications 1.The ...

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Metabolite fingerprinting: detecting biological features ...

Motivation: Metabolite fingerprinting is a technology for providing information from spectra of total compositions of metabolites. Here, spectra acquisitions by microchip-based nanoflow-direct-infusion QTOF mass spectrometry, a simple and high throughput technique, is tested for its informative power.

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The enhancement of plant secondary metabolites content in ...

2020-2-28  Plant secondary metabolites (PSM) are natural sources of biologically active compounds used for a healthy diet, in traditional medicine and in a wide range of industrial applications 1.The ...

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Metabolite fingerprinting of Punica granatum L ...

2017-1-13  Metabolite fingerprinting of Punica granatum L. (pomegranate) polyphenols by means of high-performance liquid chromatography with diode array and electrospray ionization-mass spectrometry detection. Brighenti V(1), Groothuis SF(2), Prencipe FP(1), Amir R(3), Benvenuti S(1), Pellati F(4).

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(PDF) Metabolic fingerprinting and profiling in plants ...

2014-3-18  In one approach, metabolite fingerprinting, multivariate analysis of unassigned 1H NMR spectra is used to compare the overall metabolic composition of wild-type, mutant, and transgenic plant ...

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Metabolite fingerprinting of ethyl acetate fraction of ...

2021-2-1  The present study profiled various chemical constituents from the ethyl acetate fraction of Kigelia africana fruit extract in a comparative manner using modern chromatographic methods coupled with mass spectrometric techniques.K. africana plant has a wide usage in folklore medicine for numerous ailments with the ethyl acetate fraction having high antifungal and anti-plasmodial activities.

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Metabolite fingerprinting of buckwheat in the malting ...

2020-11-17  With aims to better optimize and improve the industrial buckwheat maling process, metabolite analysis was carried out by gas chromatography–mass spectrometry (GC/MS) and high pressure liquid chromatography (HPLC) to investigate the time-dependent metabolic changes during buckwheat malting. Sixty-four metabolites, which covered a broad spectrum of polar (e.g. amino

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Pyrolysis GC-MS-based metabolite fingerprinting for ...

2010-1-1  Metabolite fingerprinting was applied in the attempt to evaluate the quality of Angelica acutiloba dried roots (Yamato-toki). A pyrolyser coupled to a gas chromatography mass spectrometer (PY-GC-MS) was used to obtain higher chemical universality by analyzing whole compounds including high molecular weight metabolites.

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Metabolomics of medicinal and aromatic plants: Goldmines ...

2021-1-1  Metabolomics is a rapidly developing technology and major approaches currently used in plant metabolomics research include metabolic fingerprinting, metabolite profiling, and targeted analysis (Shulaev, 2006). Some of the strategies used for the metabolomic study are described further:

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Plant metabolism as studied by NMR spectroscopy ...

2017-11-1  This approach is similar to plant fingerprinting, but without a time-consuming and complex step of intracellular metabolite extraction. In plant metabolomics this approach is limited to the growth medium of plant cell suspensions or of callus, and to the collection medium of root exudates (see Section 2.7 and Ref. ). Plant cells are ...

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Plant Metabolomics: A Characterisation of Plant

2018-9-25  14 Plant Metabolomics: A Characterisation of Plant Responses to Abiotic Stresses Annamaria Genga 1, Monica Mattana 1, Immacolata Coraggio 1, Franca Locatelli 1, Pietro Piffanelli 2 and Roberto Consonni 3 1Istituto di Biologia e Biotecnologia Agra ria, Consiglio Nazionale delle Ricerche 2Parco Tecnologico Padano 3Istituto per lo Studio delle Macromolecole, Laboratorio NMR,

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Metabolite fingerprinting and profiling in plants using ...

Metabolite fingerprinting by NMR is a fast, convenient, and effective tool for discriminating between groups of related samples and it identifies the most important regions of the spectrum for further analysis. In a second approach, metabolite profiling, the 1H NMR spectra of tissue extracts are assigned, a process that typically identifies 20 ...

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EP0378921A2 - Enhancement of plant metabolite production ...

Methods are provided for maximizing the production of secondary metabolites produced from plant cell cultures. The methods involve monitoring the physiological state of the culture cells so that elicitors, which stimulate metabolite production, can be applied at an optimum time. This enhanced production of metabolites following elicitation can be further maximized by the addition of cell ...

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Comprehensive LC-MS-Based Metabolite Fingerprinting ...

Herein, we present a metabolite fingerprinting workflow, which is applicable for the analysis of tissues and fluids derived from plants and fungi. This is based on a broad metabolite coverage by a two-phase extraction and the separate analysis of polar, and nonpolar compounds by positive as well as negative electrospray ionization.

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Metabolite Fingerprinting in Transgenic Nicotiana

2020-10-13  Articles Department of Plant, Soil, and Agricultural Systems 2005 Metabolite Fingerprinting in Transgenic Nicotiana tabacum Altered by the Escherichia coli Glutamate Dehydrogenase Gene R. Mungur A. D. M. Glass D. B. Goodenow David A. Lightfoot Southern Illinois

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Pyrolysis GC-MS-based metabolite fingerprinting for ...

2010-1-1  Metabolite fingerprinting was applied in the attempt to evaluate the quality of Angelica acutiloba dried roots (Yamato-toki). A pyrolyser coupled to a gas chromatography mass spectrometer (PY-GC-MS) was used to obtain higher chemical universality by analyzing whole compounds including high molecular weight metabolites.

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A review on trends in production of secondary

as plant leaves, stems, roots, meristems etc for both the ways for multiplication and extraction of secondary metabolites. Strain improvement, methods for the selection of high-producing cell lines, and medium optimizations can lead to an enhancement in secondary metabolite production. However, most

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[PDF] Predicting interpretability of metabolome models ...

In metabolome fingerprinting by mass spectrometry, each metabolite can be represented by signals at several m/z. Exploiting a prior understanding of expected biochemical differences between sample classes, we aimed to develop meaningful metrics relevant to the significance both of the overall RF model and individual, potentially explanatory ...

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Plant Metabolomics: A Characterisation of Plant

2018-9-25  14 Plant Metabolomics: A Characterisation of Plant Responses to Abiotic Stresses Annamaria Genga 1, Monica Mattana 1, Immacolata Coraggio 1, Franca Locatelli 1, Pietro Piffanelli 2 and Roberto Consonni 3 1Istituto di Biologia e Biotecnologia Agra ria, Consiglio Nazionale delle Ricerche 2Parco Tecnologico Padano 3Istituto per lo Studio delle Macromolecole, Laboratorio NMR,

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Modulation of steroidal glycoalkaloid biosynthesis in ...

Jasmonic acid (JA) is a phytohormone involved in plant development and defense. A major role of JA is the enhancement of secondary metabolite production, such as response to herbivory. Systemin is a bioactive plant peptide of 18 amino acids that contributes to the induction of local and systemic def

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Metabolic fingerprints reveal how an insect metabolome is ...

2019-2-22  Plants are used as a food source by nearly half of all insect species (Wu and Baldwin 2010), and there is a large body of research examining factors that affect these herbivore–plant interactions (Schoonhoven et al. 2005).When insect herbivores consume host plants, they take in nutritious primary plant metabolites as well as defensive secondary metabolites which can be repellent or even toxic.

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